SARS-CoV-2 Neutralizing antibody test kit (Lateral chromatography)
Cholinga Chogwiritsidwa Ntchito
SARS-CoV-2 Neutralizing antibody Test Kit (Lateral chromatography) ndiyoyenera kuzindikira mwachangu ma antibodies a SARS-CoV-2 mu seramu ya uman, plasma, kapena magazi athunthu (capillary kapena venous).Chidacho chimapangidwa ngati chothandizira kuwunika momwe chitetezo cha mthupi chimasinthira ku SARS-CoV-2.Kwa in vitro diagnostic ntchito kokha.Kugwiritsa ntchito akatswiri okha.
Mfundo Yoyesera
SARS-CoV-2 Neutralizing antibody Test Kit (Lateral chromatography) ndi njira yoyeserera yodziwikiratu yokhala ndi membrane yodziwira ma antibodies a SARS-CoV-2 RBD mu seramu, plasma ndi magazi athunthu.Chitsanzocho chimaponyedwa mu chitsime chachitsanzo ndipo chitsanzo cha dilution buffer chimawonjezeredwa pambuyo pake.Ma antibodies a SARS-CoV-2 RBD mu zitsanzo amaphatikizana ndi mapuloteni olembedwa ndi tinthu a RBD ndikupanga ma chitetezo chamthupi.Pamene zovuta zimasamukira pa nembanemba ya nitrocellulose ndi capillary action, ma antibodies a RBD amatha kugwidwa ndi mapuloteni ena a RBD atakutidwa pa malo oyesera (T mzere), kupanga mzere wa chizindikiro.Malo owongolera bwino amakutidwa ndi anti-chicken IgY, ndipo nkhuku yolembedwa tinthu tating'onoting'ono IgY imatengedwa kuti ikhale yovuta komanso yophatikiza mu mzere wa C.Ngati mzere wa C suwoneka, umasonyeza kuti zotsatira zake ndi zosavomerezeka, ndipo kuyesanso kumafunika.
Zamkatimu Zazikulu
Zigawo zoperekedwa zandandalikidwa patebulo.
| Gawo/REF | B006C-01 | B006C-25 |
| Mayeso Kaseti | 1 mayeso | 25 mayesero |
| Mowa Pad | 1 chidutswa | 25 pcs |
| Sample Diluent | 1 botolo | 25 botolo |
| Malangizo Ogwiritsa Ntchito | 1 chidutswa | 1 chidutswa |
| Lancet yotayika | 1 chidutswa | 25 ma PC |
| Chotsitsa | 1 chidutswa | 25 ma PC |
| Satifiketi Yogwirizana | 1 chidutswa | 1 chidutswa |
Operation Flow
Gawo 1: Sample
Sungani Seramu yaumunthu / Plasma / Magazi Athunthu moyenera.
Gawo 2: Kuyesa
1. Tsegulani khadi loyendera thumba la aluminium zojambulazo.Chotsani khadi yoyesera ndikuyiyika mopingasa patebulo.
2. Gwiritsani ntchito pipette yotayika, tumizani 10µL seramu / kapena 10µL plasma/ kapena 20µL magazi athunthu mu chitsime cha chitsanzo pa kaseti yoyesera.
3. Tsegulani buffer chubu pokhota kuchokera pamwamba.Gwirani botolo la bafa molunjika ndi 1 cm pamwamba pa buffer bwino.Onjezani madontho atatu (pafupifupi 100 µL) a buffer mu buffer bwino pa kaseti yoyesera.
Gawo 3: Kuwerenga
Pambuyo pa mphindi 10, werengani zotsatira zake mowonekera.(Zindikirani: chitaniOSATIwerengani zotsatira pambuyo pa mphindi 15!)
Kutanthauzira zotsatira
Zotsatira Zabwino
Ngati zonse ziwiri zowongolera khalidwe C mzere ndi mzere wa T zikuwonekera, zikutanthauza kuti ma antibodies a SARS-CoV-2 apezeka, ndipo zotsatira zake zimakhala zabwino pakuletsa ma antibodies.
Zotsatira Zoipa
Ngati mzere wa C wowongolera ukuwoneka bwino ndipo mzere wa T wozindikirika sukuwonetsa mtundu, zikutanthauza kuti ma antibodies a SARS-CoV-2 osasokoneza sanapezeke ndipo zotsatira zake ndi zoipa.
Zotsatira zosalondola
Ngati mzere wa C wowongolera khalidwe sungathe kuwonedwa, zotsatira zake ndi zosavomerezeka mosasamala kanthu kuti pali mzere wodziwikiratu, ndipo kuyesa kuyenera kubwerezedwa.
Kuitanitsa Zambiri
| Dzina lazogulitsa | Mphaka.Ayi | Kukula | Chitsanzo | Shelf Life | Trans.ndi Sto.Temp. |
| SARS-CoV-2 Neutralizing antibody test kit (Lateral chromatography) | B006C-01 | 1 mayeso / zida | S/P/WB | 18 Miyezi | 2-30 ℃ / 36-86 ℉ |
| B006C-25 | 25 mayeso / zida |
