typhoid IgG/IgM Antibody test kit (Immunochromatographic Assay)
Zambiri zamalonda
Cholinga Chogwiritsidwa Ntchito
typhoid IgG/IgM Antibody Test Kit (Immunochromatographic Assay) imatenga njira ya golidi ya colloidal kuti izindikire bwino antibody ya typhoid bacillus (lipopolysaccharide antigen ndi antigen yakunja ya membrane protein antigen) mu seramu yamunthu / plasma, yomwe ili yoyenera kuzindikira koyambirira kwa typhoid. .
Mfundo Yoyesera
typhoid IgG/IgM Antibody Test Kit (Immunochromatographic Assay) ndi lateral flow chromatography immunoassay.Kaseti yoyesera imakhala ndi: 1) pad yamtundu wa burgundy conjugate yomwe ili ndi antigen ya S. typhoid H antigen ndi O antigen yophatikizidwa ndi golide wa colloid (typhoid conjugates) ndi ma conjugates a kalulu IgG golide, 2) mzere wa nitrocellulose wokhala ndi magulu awiri oyesera (Ig). ndi magulu a IgM) ndi gulu lowongolera (C band).Gulu la IgM limakutidwa kale ndi monoclonal anti-human IgM kuti azindikire IgM anti-S.typhi, gulu la IgG limakutidwa kale ndi ma reagents kuti azindikire za IgG anti-S.typhi, ndipo gulu la C limakutidwa kale ndi anti rabbit IgG.
Zamkatimu Zazikulu
Zigawo zoperekedwa zandandalikidwa patebulo.
| Zipangizo / zoperekedwa | Kuchuluka (1 Mayeso/Kiti)
| Kuchuluka(5Mayeso/Kiti)
| Kuchuluka(25Mayeso/Kit)
|
| Zida Zoyesera | 1 mayeso | 5 mayeso | 25 mayesero |
| Bafa | 1 botolo | 5 botolo | 25/2 mabotolo |
| Chotsitsa | 1 chidutswa | 5 gawo | 25 gawo |
| Chikwama Chonyamula Chitsanzo | 1 chidutswa | 5 pcs | 25 pcs |
| Lancet yotayika | 1 chidutswa | 5 pcs | 25 pcs |
| Malangizo Ogwiritsa Ntchito | 1 chidutswa | 1 chidutswa | 1 chidutswa |
| Satifiketi Yogwirizana | 1 chidutswa | 1 chidutswa | 1 chidutswa |
Operation Flow
Gawo 1: Sample
Sungani Seramu yaumunthu / Plasma / Magazi Athunthu moyenera.
Gawo 2: Kuyesa
1. Chotsani chubu chochotsa muzitsulo ndi bokosi loyesera kuchokera m'thumba la filimu pong'amba mphako.Tsegulani thumba loyendera thumba la aluminium zojambulazo.Chotsani khadi yoyesera ndikuyiyika mopingasa patebulo.
2. Gwiritsani ntchito pipette yotayika, tumizani 4μL seramu (kapena plasma), kapena 4μL magazi athunthu mu chitsime cha chitsanzo pa kaseti yoyesera.
3.Tsegulani buffer chubu pokhota kuchokera pamwamba.Ikani madontho atatu (pafupifupi 80 μL) oyezera muyeso woyezera wozungulira wozungulira.Yambani kuwerenga.
Gawo 3: Kuwerenga
Werengani zotsatira pa 15 min.Zotsatira pambuyo pa mphindi 20 ndizolakwika.
Kutanthauzira zotsatira
Zotsatira zoyipa
Chingwe chowongolera khalidwe C chokha chimawonekera ndipo mizere yodziwira G ndi M sikuwonetsa, zikutanthauza kuti palibe antibody ya Typhoid yomwe imadziwika ndipo zotsatira zake zimakhala zoipa.
Zotsatira Zabwino
Ngati zonse mzere wowongolera mtundu C ndi mzere wozindikira M ziwoneka= antibody ya typhoid IgM yazindikirika, ndipo zotsatira zake zimakhala zabwino kwa antibody ya IgM.
Ngati zonse mzere wowongolera mtundu C ndi mzere wozindikira G zikuwoneka= antibody ya Typhoid IgG yazindikirika ndipo zotsatira zake zimakhala zabwino kwa antibody IgG.
Ngati onse awiri mzere wowongolera khalidwe C ndi mizere yodziwira G ndi M ikuwoneka= ma typhoid IgG ndi IgM apezeka, ndipo zotsatira zake zimakhala zabwino kwa ma antibodies a IgG ndi IgM.
Zotsatira zosalondola
Ngati mzere wowongolera khalidwe C sungathe kuwonedwa, zotsatira zidzakhala zosavomerezeka
mosasamala kanthu kuti mzere woyesera ukuwonetsa, ndipo kuyesa kuyenera kubwerezedwa.
Kuitanitsa Zambiri
| Dzina lazogulitsa | Mphaka.Ayi | Kukula | Chitsanzo | Shelf Life | Trans.ndi Sto.Temp. |
| Typhoid IgG/IgM Antibody test kit (Immunochromatographic Assay) | B023C-01 | 1 mayeso / zida | Seramu / Plasma / Magazi Athunthu | 18 Miyezi | 2-30 ℃ / 36-86 ℉ |
| B023C-05 | 5 mayeso / zida | ||||
| B023C-25 | 25 mayeso / zida |
