Chida choyesera ma antibodies a Typhoid IgG/IgM (Immunochromatographic Assay) Chithunzi Chowonetsedwa

Kiti yoyesera ya Typhoid IgG/IgM Antibody (Kuyesa kwa Immunochromatographic)

Kufotokozera Kwachidule:

Chitsanzo	Seramu/Magazi a m'magazi/Magazi Athunthu	Mtundu	Kaseti
Kuzindikira	97.93%	Kufotokozera Mwapadera	98.70%
Kusinthasintha ndi Kusinthasintha kwa Kutentha.	2-30℃ / 36-86℉	Nthawi Yoyesera	Mphindi 15
Kufotokozera	1 Mayeso/Chida 5 Mayeso/Chida 25 Mayeso/Chida

Tumizani imelo kwa ife

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Tsatanetsatane wa malonda

Kugwiritsa Ntchito Koyenera
Chida Choyesera Matenda a Typhoid IgG/IgM Antibody (Immunochromatographic Assay) chimagwiritsa ntchito njira ya colloidal gold kuti chizindikire bwino momwe ma antibodies a typhoid bacillus (lipopolysaccharide antigen ndi external membrane protein antigen) alili mu seramu/plasma ya anthu, yomwe ndi yoyenera kuzindikira matenda a typhoid koyambirira.

Mfundo Yoyesera
Chida Choyesera Matenda a Typhoid IgG/IgM Antibody (Immunochromatographic Assay) ndi njira yoyesera matenda a chromatography. Kaseti yoyeserayi ili ndi: 1) cholumikizira chamtundu wa burgundy chokhala ndi recombinant S. typhoid H antigen ndi O antigen cholumikizidwa ndi golide wa colloid (Typhoid conjugates) ndi zolumikizira za kalulu za IgG-golide, 2) mzere wa nitrocellulose membrane wokhala ndi magulu awiri oyesera (IgG ndi IgM bands) ndi gulu lowongolera (C band). Gulu la IgM limapakidwa kale ndi monoclonal anti-human IgM kuti lizindikire IgM anti-S. typhi, gulu la IgG limapakidwa kale ndi ma reagents kuti lizindikire IgG anti-S.typhi, ndipo gulu la C limapakidwa kale ndi mbuzi anti-kalulu IgG.

Zamkatimu Zazikulu

Zigawo zomwe zaperekedwa zalembedwa patebulo.

Zipangizo / zoperekedwa	Kuchuluka (Mayeso/Chida chimodzi)	Kuchuluka (Mayeso 5/Zida)	Kuchuluka (Mayeso 25/Chida)
Zida Zoyesera	Mayeso 1	Mayeso 5	Mayeso 25
Buffer	Botolo limodzi	Mabotolo 5	Mabotolo 25/2
Dothi lotsitsa	Chidutswa chimodzi	Chidutswa 5	Zidutswa 25
Chikwama Choyendera cha Zitsanzo	Chidutswa chimodzi	Ma PC 5	Ma PC 25
Lancet Yotayidwa	Chidutswa chimodzi	Ma PC 5	Ma PC 25
Malangizo Ogwiritsira Ntchito	Chidutswa chimodzi	Chidutswa chimodzi	Chidutswa chimodzi
Satifiketi Yotsatira Malamulo	Chidutswa chimodzi	Chidutswa chimodzi	Chidutswa chimodzi

Kuyenda kwa Ntchito

Gawo 1: Kusankha Zitsanzo

Kusonkhanitsa Seramu/Magazi a m'magazi/magazi athunthu a munthu moyenera.

Gawo 2: Kuyesa

1. Chotsani chubu chochotseramo zinthu kuchokera mu kit ndi bokosi loyesera kuchokera mu thumba la filimu pong'amba cholumikizira. Tsegulani thumba la pepala la aluminiyamu la khadi lowunikira. Chotsani khadi loyesera ndi kuliyika mopingasa patebulo.

2. Gwiritsani ntchito pipette yotayidwa, tumizani seramu ya 4μL (kapena plasma), kapena magazi athunthu a 4μL mu chitsime cha chitsanzo chomwe chili pa kaseti yoyesera.

3. Tsegulani chubu chosungira madzi popotoza pamwamba. Ikani madontho atatu (pafupifupi 80 μL) a choyezera madzi mu choyezera madzi chokhala ndi mawonekedwe ozungulira. Yambani kuwerengera.

Gawo 3: Kuwerenga

Werengani zotsatira pa mphindi 15. Zotsatira pambuyo pa mphindi 20 sizolondola.

Kutanthauzira Zotsatira

Zotsatira zoipa
Mzere wowongolera khalidwe C wokha ndi womwe umawonekera ndipo mizere yozindikira G ndi M siikuonekera, zikutanthauza kuti palibe antibody ya Typhoid yomwe yapezeka ndipo zotsatira zake zimakhala zoipa.

Zotsatira Zabwino
Ngati mzere wowongolera khalidwe C ndi mzere wozindikira M zikuwonekera = antibody ya Typhoid IgM yapezeka, ndipo zotsatira zake zimakhala zabwino kwa antibody ya IgM.
Ngati mzere wowongolera khalidwe C ndi mzere wozindikira G zikuwonekera = ma antibodies a Typhoid IgG apezeka ndipo zotsatira zake zimakhala zabwino kwa ma antibodies a IgG.
Ngati mzere wowongolera khalidwe C ndi mizere yozindikira G ndi M zikuwonekera = ma antibodies a Typhoid IgG ndi IgM apezeka, ndipo zotsatira zake zimakhala zabwino kwa ma antibodies onse a IgG ndi IgM.

Zotsatira Zosalondola
Ngati mzere wowongolera khalidwe C sungawonekere, zotsatira zake zidzakhala zosavomerezeka
kaya mzere woyesera ukuwoneka, ndipo mayesowo ayenera kubwerezedwanso.

Zambiri Zokhudza Oda

Dzina la Chinthu	Mphaka. Ayi	Kukula	Chitsanzo	Moyo wa Shelufu	Kusinthasintha ndi Kusinthasintha kwa Kutentha.
Matenda a typhoid Kiti yoyesera ya IgG/IgM Antibody (Kuyesa kwa Immunochromatographic)	B023C-01	1 mayeso/zida	Seramu/Magazi a m'magazi/Magazi Athunthu	Miyezi 18	2-30℃ / 36-86℉
	B023C-05	Mayeso 5/zida
	B023C-25	Mayeso 25/zida