Zipangizo zoyesera mwachangu za H. pylori antigen

Zipangizo zoyesera mwachangu za H. pylori antigen,
mayeso a ndowe ya h pylori, H. Pylori Antigen, Njuchi ya H. pylori, H. pylori ali ndi kachilombo, Kuyesa kwa H. Pylori,
Kugwiritsa Ntchito Koyenera

H. Pylori AntigenKiti Yoyesera Mwachangu (Lateral chromatography) iyenera kugwiritsidwa ntchito pofufuza helicobacter pylori antigen mu ndowe za anthu mu vitro. Mayesowa angagwiritsidwe ntchito ndi akatswiri azachipatala okha.

Mfundo Yoyesera

Kitiyi ndi ya immunochromatographic ndipo imagwiritsa ntchito njira ya sandwich ya antibody iwiri kuti izindikire H. Pylori Antigen. Ili ndi tinthu tating'onoting'ono tokhala ndi zilembo za H. Pylori monoclonal antibody zomwe zakulungidwa mu conjugate pad. Antibody ina ya H. Pylori monoclonal yomwe imakhazikika pa membrane ya NC. ndi mzere wowongolera khalidwe C womwe umakutidwa ndi antibody ya mbewa ya IgG, imagwiritsa ntchito antigen-antibody reaction yapadera kwambiri komanso ukadaulo wa lateral chromatography, zomwe zimatsimikizira kuchuluka kwa H. Pylori Antigen m'chimbudzi cha anthu.

Zamkatimu Zazikulu

Zigawo zomwe zaperekedwa zalembedwa patebulo.

Kuyenda kwa Ntchito

Gawo 1: Kusankha Zitsanzo

Sungani zidebe zoyera komanso zosatulutsa madzi kuchokera ku fecalspecimensin.

Gawo 2: Kuyesa

1. Chotsani kaseti yoyesera kuchokera mu thumba la zojambulazo ndikuyiyika pamalo athyathyathya

2. Tulutsani botolo la chitsanzo, gwiritsani ntchito ndodo yolumikizira yomwe yaikidwa pa chivundikiro kuti mutumize kachidutswa kakang'ono ka chitsanzo cha ndowe (3-5 mm m'mimba mwake; pafupifupi 30-50 mg) mu botolo la chitsanzo lomwe lili ndi chosungira chokonzekera chitsanzo.

3. Bwezerani ndodo mu botolo ndikulilimbitsa bwino. Sakanizani chitsanzo cha ndowe ndi chosungiramo madzi bwino pogwedeza botolo kangapo ndikusiya chubucho kwa mphindi ziwiri.

4. Tsegulani nsonga ya botolo la chitsanzo ndikugwirizira botolo moyimirira pamwamba pa chitsime cha chitsanzo cha Kaseti, perekani madontho atatu (pafupifupi 100 -120μL) a chitsanzo cha ndowe chochepetsedwa ku chitsime cha chitsanzo.

Gawo 3: Kuwerenga

Patatha mphindi 15, werengani zotsatirazo m'maso. (Dziwani: MUSAWERENGE zotsatirazo patatha mphindi 20!)

Kutanthauzira Zotsatira

1.Zotsatira zoipa

Ngati mzere wowongolera khalidwe C wokha uonekera ndipo mizere yozindikira G ndi M siionekera, zikutanthauza kuti palibe antibody yatsopano ya coronavirus yomwe yapezeka ndipo zotsatira zake zimakhala zoipa.

2. Zotsatira Zabwino

2.1 Ngati mzere wowongolera khalidwe C ndi mzere wozindikira M zonse zikuwonekera, zikutanthauza kuti antibody yatsopano ya coronavirus IgM yapezeka, ndipo zotsatira zake zimakhala zabwino kwa antibody ya IgM.

2.2 Ngati mzere wowongolera khalidwe C ndi mzere wozindikira G zonse zikuwonekera, zikutanthauza kuti antibody yatsopano ya coronavirus IgG yapezeka ndipo zotsatira zake zimakhala zabwino kwa antibody ya IgG.

2.3 Ngati mzere wowongolera khalidwe C ndi mizere yozindikira G ndi M zikuwonekera, zikutanthauza kuti ma antibodies atsopano a coronavirus IgG ndi IgM apezeka, ndipo zotsatira zake zimakhala zabwino kwa ma antibodies onse a IgG ndi IgM.

3. Zotsatira Zolakwika

Ngati mzere wowongolera khalidwe C sungawonekere, zotsatira zake zidzakhala zosavomerezeka mosasamala kanthu kuti mzere woyesera waonekera kapena ayi, ndipo mayesowo ayenera kubwerezedwanso.

Zambiri Zokhudza Oda

Kiti Yoyesera ya H. Pylori Antigen

H. pylori ndi bakiteriya wooneka ngati spiral yemwe angayambitse zilonda ndi kutupa kwa matumbo a m'mimba, zomwe zingayambitse khansa ya m'mimba.

Chida Choyesera cha H. Pylori Antigen ndi njira yosavuta yoyesera mabakiteriyawa, zomwe zingatheke kuchokera kunyumba kwanu.

Chidacho chili ndi swab, lancet ndi botolo la mankhwala osungira omwe mumagwiritsa ntchito kusonkhanitsa chitsanzo cha malovu kapena ndowe zanu. Swab ndi test stick zimayikidwa mu botolo, kusakaniza ndi kusungidwa kutentha kwa chipinda kwa mphindi 10. Zotsatira zake zimawonekera patangopita mphindi 10 kuchokera pamene mwaikamo botolo.