Mayeso Ofulumira a H. pylori Stool Antigen

Mayeso Ofulumira a H. pylori Stool Antigen,
H. Pylori Antigen, H. pylori baterial, H. pylori ali ndi kachilombo, Kuyesa kwa H. Pylori, Helicobacter pylori fecal antigen,
Kugwiritsa Ntchito Koyenera

H. Pylori AntigenKiti Yoyesera Mwachangu (Lateral chromatography) iyenera kugwiritsidwa ntchito pofufuza helicobacter pylori antigen mu ndowe za anthu mu vitro. Mayesowa angagwiritsidwe ntchito ndi akatswiri azachipatala okha.

Mfundo Yoyesera

Chidachi chili ndi immunochromatographic ndipo chimagwiritsa ntchito njira ya sandwich ya antibody iwiri kuti chizindikireH. Pylori AntigenIli ndi tinthu tating'onoting'ono tozungulira tolembedwa kuti H. Pylori monoclonal antibody tomwe takulungidwa mu conjugate pad. Antibody ina ya H. Pylori monoclonal yomwe imakhazikika pa nembanemba ya NC. ndi mzere wowongolera khalidwe C womwe waphimbidwa ndi antibody ya mbewa ya IgG, imagwiritsa ntchito antigen-antibody reaction yapadera kwambiri komanso ukadaulo wa lateral chromatography, zomwe zimatsimikizira kuchuluka kwa H. Pylori Antigen m'chimbudzi cha anthu.

Zamkatimu Zazikulu

Zigawo zomwe zaperekedwa zalembedwa patebulo.

Kuyenda kwa Ntchito

Gawo 1: Kusankha Zitsanzo

Sungani zidebe zoyera komanso zosatulutsa madzi kuchokera ku fecalspecimensin.

Gawo 2: Kuyesa

1. Chotsani kaseti yoyesera kuchokera mu thumba la zojambulazo ndikuyiyika pamalo athyathyathya

2. Tulutsani botolo la chitsanzo, gwiritsani ntchito ndodo yolumikizira yomwe yaikidwa pa chivundikiro kuti mutumize kachidutswa kakang'ono ka chitsanzo cha ndowe (3-5 mm m'mimba mwake; pafupifupi 30-50 mg) mu botolo la chitsanzo lomwe lili ndi chosungira chokonzekera chitsanzo.

3. Bwezerani ndodo mu botolo ndikulilimbitsa bwino. Sakanizani chitsanzo cha ndowe ndi chosungiramo madzi bwino pogwedeza botolo kangapo ndikusiya chubucho kwa mphindi ziwiri.

4. Tsegulani nsonga ya botolo la chitsanzo ndikugwirizira botolo moyimirira pamwamba pa chitsime cha chitsanzo cha Kaseti, perekani madontho atatu (pafupifupi 100 -120μL) a chitsanzo cha ndowe chochepetsedwa ku chitsime cha chitsanzo.

Gawo 3: Kuwerenga

Patatha mphindi 15, werengani zotsatirazo m'maso. (Dziwani: MUSAWERENGE zotsatirazo patatha mphindi 20!)

Kutanthauzira Zotsatira

1.Zotsatira zoipa

Ngati mzere wowongolera khalidwe C wokha uonekera ndipo mizere yozindikira G ndi M siionekera, zikutanthauza kuti palibe antibody yatsopano ya coronavirus yomwe yapezeka ndipo zotsatira zake zimakhala zoipa.

2. Zotsatira Zabwino

2.1 Ngati mzere wowongolera khalidwe C ndi mzere wozindikira M zonse zikuwonekera, zikutanthauza kuti antibody yatsopano ya coronavirus IgM yapezeka, ndipo zotsatira zake zimakhala zabwino kwa antibody ya IgM.

2.2 Ngati mzere wowongolera khalidwe C ndi mzere wozindikira G zonse zikuwonekera, zikutanthauza kuti antibody yatsopano ya coronavirus IgG yapezeka ndipo zotsatira zake zimakhala zabwino kwa antibody ya IgG.

2.3 Ngati mzere wowongolera khalidwe C ndi mizere yozindikira G ndi M zikuwonekera, zikutanthauza kuti ma antibodies atsopano a coronavirus IgG ndi IgM apezeka, ndipo zotsatira zake zimakhala zabwino kwa ma antibodies onse a IgG ndi IgM.

3. Zotsatira Zolakwika

Ngati mzere wowongolera khalidwe C sungawonekere, zotsatira zake zidzakhala zosavomerezeka mosasamala kanthu kuti mzere woyesera waonekera kapena ayi, ndipo mayesowo ayenera kubwerezedwanso.

Zambiri Zokhudza Oda

H. pylori ndi bakiteriya yomwe imatha kufalikira m'mimba. Ndi imodzi mwa matenda omwe amapezeka kwambiri m'mimba komanso m'mimba.

Mayeso a H. pylori antigen amatsimikizira ma antibodies ku mabakiteriya omwe ali m'magazi mwanu. Ngati mwadwala H. pylori, ma antibodies amenewa adzakhalapo m'magazi mwanu.