Kiti Yoyesera Mwachangu ya Monkeypox Virus IgM/IgG Antibody (Lateral Chromatography)
Tsatanetsatane wa malonda
Kugwiritsa Ntchito Koyenera
Chida Choyesera Chofulumira cha Monkeypox Virus IgM/IgG Antibody chimagwiritsidwa ntchito pozindikira bwino ma antibodies a Monkeypox Virus IgM/IgG mu seramu ya anthu, plasma kapena magazi athunthu. Cholinga chake ndi kugwiritsa ntchito poyesa matenda m'thupi, komanso kugwiritsidwa ntchito ndi akatswiri okha.
Mfundo Yoyesera
Chipangizo choyesera cha Monkeypox Virus IgM/IgG chili ndi mizere itatu yophimbidwa kale, "G" (Monkeypox IgG Test Line), "M" (Monkeypox IgM Test Line) ndi "C" (Control Line) pamwamba pa nembanemba. "Control Line" imagwiritsidwa ntchito poyang'anira njira. Chitsanzo chikawonjezedwa pachitsime cha chitsanzo, ma anti-Monkeypox IgGs ndi ma IgM omwe ali mu chitsanzocho amakumana ndi mapuloteni obwezeretsanso a Monkeypox virus envelope omwe amalumikizana ndikupanga antibody-antigen complex. Pamene complex ikuyenda motsatira chipangizo choyesera kudzera mu capillary action, idzagwidwa ndi anti-human IgG ndi anti-human IgM yomwe imalephera kuyenda m'mizere iwiri yoyesera kudutsa chipangizo choyesera ndikupanga mzere wamtundu. Kuti ikhale ngati njira yowongolera njira, mzere wamtundu nthawi zonse umawonekera m'dera la mzere wowongolera, kusonyeza kuti kuchuluka koyenera kwa chitsanzo kwawonjezeredwa ndipo kupukuta kwa nembanemba kwachitika.
Zamkatimu Zazikulu
Zigawo zomwe zaperekedwa zalembedwa patebulo.
| Chigawo cha REFREF | B030C-01 | B030C-05 | B030C-25 |
| Kaseti Yoyesera | Mayeso 1 | Mayeso 5 | Mayeso 25 |
| Chitsanzo cha Diluent | Botolo limodzi | Mabotolo 5 | Mabotolo 25 |
| Lancet Yotayidwa | Chidutswa chimodzi | Ma PC 5 | Ma PC 25 |
| Chophimba cha Mowa | Chidutswa chimodzi | Ma PC 5 | Ma PC 25 |
| Chotsitsa Chotayidwa | Chidutswa chimodzi | Ma PC 5 | Ma PC 25 |
| Malangizo Ogwiritsira Ntchito | Chidutswa chimodzi | Chidutswa chimodzi | Chidutswa chimodzi |
| Satifiketi Yotsatira Malamulo | Chidutswa chimodzi | Chidutswa chimodzi | Chidutswa chimodzi |
Kuyenda kwa Ntchito
- Gawo 1: Kusankha Zitsanzo
Kusonkhanitsa Seramu/Magazi a m'magazi/magazi athunthu a munthu moyenera.
- Gawo 2: Kuyesa
1. Mukakonzeka kuyesa, tsegulani thumba lomwe lili pa notch ndikuchotsa chipangizocho.
chipangizo choyesera chili pamalo oyera komanso athyathyathya.
2. Dzazani chitsanzo cha dropper ya pulasitiki. Gwirani dropper moyimirira,
perekani 10µL ya seramu/plasma kapena 20µL ya magazi athunthu mu chitsime cha chitsanzo,
kuonetsetsa kuti palibe thovu la mpweya.
3. Onjezani madontho atatu (pafupifupi 100 µL) a chitsanzo chosungunula kuti muyese bwino ndi
botolo litayikidwa moyimirira. Yambani kuwerenga.
- Gawo 3: Kuwerenga
Patatha mphindi 15, werengani zotsatirazo m'maso. (Dziwani: MUSAWERENGE zotsatirazo patatha mphindi 20!)
Kutanthauzira Zotsatira
| Zabwino | Zoyipa | Chosalondola | ||
| -Zotsatira Zabwino za IgM- Mzere wowongolera (C) ndi mzere wa IgM (M) zimawonekera pa chipangizo choyesera. Izi ndi ali ndi ma antibodies a IgM ku kachilombo ka monkeypox. | -Zotsatira Zabwino za IgG- Mzere wowongolera (C) ndi mzere wa IgG (G) zimawonekera pa chipangizo choyesera. Izi zikutanthauza kuti pali ma antibodies a IgG ku kachilombo ka monkeypox. | -IgM Yabwino& IgG- Mzere wowongolera (C), IgM (M) ndi mzere wa IgG (G) zimawonekera pa chipangizo choyesera. Izi ndi zabwino kwa ma antibodies onse a IgM ndi IgG. | Mzere wa C wokha ndi womwe umawonekera ndipo mzere wa G ndi mzere wa M sizimawonekera. | Palibe mzere womwe umawonekera mu mzere wa C ngakhale mzere wa G ndi/kapena mzere wa M ukuwonekera kapena ayi. |
Zambiri Zokhudza Oda
| Dzina la Chinthu | Mphaka. Ayi | Kukula | Chitsanzo | Moyo wa Shelufu | Kusinthasintha ndi Kusinthasintha kwa Kutentha. |
| Kiti Yoyesera Mwachangu ya Monkeypox Virus IgM/IgG Antibody (Lateral)Chromatography) | B030C-01 | Chiyeso chimodzi/zida | S/P/WB | Miyezi 24 | 2-30℃ |
| B030C-05 | Chiyeso chimodzi/zida | ||||
| B009C-5 | Mayeso 25/zida |
