Cardiac Troponin I Rapid Test Kit (Lateral Chromatography)
Zambiri zamalonda
Cholinga Chogwiritsidwa Ntchito:
Cardiac Troponin I Rapid Test Kit imagwiritsa ntchito colloidal gold immunochromatography kuti izindikire mtima wa Troponin I (cTnI) mu seramu, madzi a m'magazi kapena magazi athunthu moyenerera kapena mopanda malire ndi khadi yokhazikika ya colorimetric.Mayesowa amagwiritsidwa ntchito ngati chithandizo pozindikira kuvulala kwa myocardial monga Acute Myocardial Infarction, Unstable Angina, Acute Myocarditis ndi Acute Coronary Syndrome.
Mfundo Zoyesa:
Cardiac Troponin I Rapid Test Kit (Lateral Chromatography) ndi mtundu kapena semi-quantitative, membrane based immunoassay pozindikira mtima wa Troponin I(cTnI) m'magazi athunthu, seramu kapena plasma.Munjira yoyesera iyi, reagent yojambulira imakhala yosasunthika mugawo la mayeso.Chitsanzocho chikawonjezedwa kudera lachitsanzo la makaseti, chimachita ndi tinthu tating'ono tomwe timakutidwa ndi anti-cTnI pamayesero.Kusakaniza uku kumasuntha motengera kutalika kwa mayeso ndikulumikizana ndi reagent ya immobilized Capture.Mawonekedwe oyesera amatha kuzindikira mtima wa Troponin I(cTnI) m'matsanzo.Ngati chitsanzocho chili ndi mtima wa Troponin I(cTnI), mzere wachikuda udzawonekera m'chigawo cha mzere woyesera ndipo kukula kwamtundu wa mzere woyesera kumawonjezeka molingana ndi chiwerengero cha cTnI, kusonyeza zotsatira zabwino.Ngati chitsanzocho chilibe mtima Troponin I(cTnI), mzere wachikuda sudzawonekera m'derali, zomwe zikuwonetsa zotsatira zoyipa.Kuti ikhale yoyang'anira ndondomeko, mzere wachikuda udzawonekera nthawi zonse m'chigawo cha mzere wolamulira, kusonyeza kuti chiwerengero choyenera cha chitsanzo chawonjezeredwa ndipo kupukuta kwa membrane kwachitika.
Zamkatimu Zazikulu
Zigawo zoperekedwa zandandalikidwa patebulo.
| Gawo REF REF | B032C-01 | B032C-25 |
| Kaseti yoyesera | 1 mayeso | 25 mayesero |
| Sample diluent | 1 botolo | 1 botolo |
| Chotsitsa | 1 chidutswa | 25 pcs |
| Standard colorimetric khadi | 1 chidutswa | 1 chidutswa |
| Satifiketi Yogwirizana | 1 chidutswa | 1 chidutswa |
Operation Flow
Gawo 1: Kukonzekera Zitsanzo
1. Zida zoyesera zimatha kuchitidwa pogwiritsa ntchito magazi athunthu, seramu kapena plasma.Ganizirani kusankha seramu kapena plasma ngati chitsanzo choyesera.Ngati musankha magazi athunthu ngati mayeso, ayenera kugwiritsidwa ntchito limodzi ndi mankhwala osungunula magazi.
2. Yesani chitsanzo pa khadi loyesera mwamsanga.Ngati kuyezetsa sikungatheke nthawi yomweyo, seramu ndi madzi a m'magazi amayenera kusungidwa mpaka masiku 7 pa 2 ~ 8 ℃ kapena kusungidwa pa -20 ℃ kwa miyezi 6 (chitsanzo chonse cha magazi chiyenera kusungidwa kwa masiku atatu pa 2 ~ 8 ℃ ) mpaka ayesedwe.
3. Zitsanzo ziyenera kubwezeretsedwanso kutentha kwa chipinda musanayesedwe.Zitsanzo zozizira zimafunika kusungunuka kwathunthu ndikusakanikirana bwino musanayesedwe, kupewa kuzizira mobwerezabwereza ndi kusungunuka.
4. Pewani kutentha zitsanzo, zomwe zingayambitse hemolysis ndi mapuloteni denaturation.Ndi bwino kupewa kugwiritsa ntchito kwambiri hemolyzed chitsanzo.Ngati sampuli ikuwoneka kuti ili ndi hemolyzed kwambiri, chitsanzo china chiyenera kupezedwa ndikuyesedwa.
Gawo 2: Kuyesa
1. Chonde werengani bukuli mosamala musanayese, bwezeretsani chitsanzo, khadi yoyezetsa ndi magazi omwe amasungunuka kutentha kwa chipinda ndi nambala ya khadi.Limbikitsani kuti mutsegule chikwama chojambulacho chikayamba kutentha komanso kugwiritsa ntchito khadi loyesera nthawi yomweyo.
2. Ikani khadi loyesera pa tebulo loyera, loyikidwa mopingasa.
Kwa zitsanzo za Serum kapena Plasma:
Gwirani chotsitsacho molunjika ndikusuntha madontho atatu a seramu kapena plasma (pafupifupi 80 L, Pipette angagwiritsidwe ntchito mwadzidzidzi) pachitsanzo chabwino, ndikuyamba chowerengera.Onani chithunzi pansipa.
Kwa chitsanzo cha Magazi Athunthu:
Gwirani chotsitsacho molunjika ndikusamutsa madontho atatu amagazi athunthu (pafupifupi 80 L) kupita pachitsanzocho bwino, kenaka yikani dontho limodzi la Sample diluent (pafupifupi 40 L), ndikuyamba chowerengera.Onani chithunzi pansipa.
Gawo 3: Kuwerenga
Pakadutsa mphindi 10-30, pezani zotsatira zowerengera molingana ndi makadi amtundu wamtundu ndi maso.
Kutanthauzira zotsatira
Zovomerezeka: Mzere umodzi wofiyira wofiyira ukuwonekera pamzere wowongolera (C).Pazotsatira zomveka, mutha kupeza semi-quantitative ndi maso okhala ndi khadi yodziwika bwino ya colorimetric:
Colour Intensity vs Reference Concentration
| Mtundu Wamphamvu | Kuyikira Kwambiri (ng / ml) |
| - | ＜0.5 |
| +- | 0.5-1 |
| + | 1~5 |
| + + | 5-15 |
| + + + | 15-30 |
| ++++ | 30-50 |
| ++++ | ＞50 |
Zosalondola: Palibe purplish red streak yomwe ikuwonekera pamzere wowongolera (C) .Izi zikutanthauza kuti machitidwe ena ayenera kukhala olakwika kapena khadi yoyeserera ndiyosavomerezeka kale.Zikatere chonde werenganinso bukuli mosamala, ndipo yesaninso ndi kaseti yatsopano yoyesera. Ngati zomwezi zidachitikanso, muyenera kusiya kugwiritsa ntchito gululi nthawi yomweyo ndikulumikizana ndi omwe akukugulirani.
Kuitanitsa Zambiri
| Dzina lazogulitsa | Mphaka.Ayi | Kukula | Chitsanzo | Shelf Life | Trans.ndi Sto.Temp. |
| Cardiac Troponin I Rapid Test Kit (Lateral Chromatography) | B032C-01 | 1 mayeso / zida | S/P/WB | Miyezi 24 | 2-30 ℃ |
| B032C-25 | 25 mayeso / zida |