Chida Choyesera Mwachangu cha Troponin I cha Mtima (Lateral Chromatography)

Kufotokozera Kwachidule:

Chitsanzo	Seramu/Magazi a m'magazi/Magazi Athunthu	Mtundu	Kaseti
Kuzindikira	99.60%	Kufotokozera Mwapadera	98.08%
Kusinthasintha ndi Kusinthasintha kwa Kutentha.	2-30℃ / 36-86℉	Nthawi Yoyesera	Mphindi 10-30
Kufotokozera	Chiyeso chimodzi/Chida; Mayeso 25/Chida

Tumizani imelo kwa ife

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Tsatanetsatane wa malonda

Ntchito Yoyenera:

Cardiac Troponin I Rapid Test Kit imagwiritsa ntchito colloidal gold immunochromatography kuti izindikire cardiac Troponin I (cTnI) mu seramu, plasma kapena magazi athunthu molingana ndi mtundu kapena kuchuluka kwake pogwiritsa ntchito khadi yodziwika bwino ya colorimetric. Mayesowa amagwiritsidwa ntchito ngati chithandizo pozindikira kuvulala kwa mtima monga Acute Myocardial Infarction, Unstable Angina, Acute Myocarditis ndi Acute Coronary Syndrome.

Mfundo Zoyesera:

Chida Choyesera cha Cardiac Troponin I (Lateral Chromatography) ndi njira yoyesera ya immunoassay yodziwika bwino kapena yocheperako, yochokera ku nembanemba kuti izindikire Troponin I(cTnI) ya mtima m'magazi athunthu, seramu kapena plasma. Mu njira yoyeserayi, reagent yojambulira imalephereka kuyenda m'dera la mzere woyesera wa mayesowo. Chitsanzocho chikawonjezedwa kudera la chitsanzo cha kaseti, chimayanjanitsidwa ndi tinthu ta anti-cTnI antibody tomwe timaphimbidwa mu mayesowo. Chosakanizachi chimasuntha mozungulira kutalika kwa mayesowo ndipo chimagwirizana ndi reagent yojambulira yosagwedezeka. Mtundu wa mayesowo ukhoza kuzindikira Troponin I(cTnI) ya mtima m'zitsanzo. Ngati chitsanzocho chili ndi Troponin I(cTnI ya mtima), mzere wamtundu udzawonekera m'dera la mzere woyesera ndipo mphamvu ya mzere woyeserayo imawonjezeka molingana ndi kuchuluka kwa cTnI, kusonyeza zotsatira zabwino. Ngati chitsanzocho chilibe Troponin I(cTnI ya mtima), mzere wamtundu sudzawonekera m'derali, kusonyeza zotsatira zoyipa. Kuti zigwire ntchito ngati njira yowongolera, mzere wofiirira nthawi zonse umawonekera m'dera la mzere wowongolera, kusonyeza kuti kuchuluka koyenera kwa zitsanzo zawonjezedwa ndipo kukanikiza kwa nembanemba kwachitika.

Zamkatimu Zazikulu

Zigawo zomwe zaperekedwa zalembedwa patebulo.

Chigawo cha REF REF	B032C-01	B032C-25
Kaseti yoyesera	Mayeso 1	Mayeso 25
Chitsanzo cha kusungunula	Botolo limodzi	Botolo limodzi
Dothi lotsitsa	Chidutswa chimodzi	Ma PC 25
Khadi lodziwika bwino la colorimetric	Chidutswa chimodzi	Chidutswa chimodzi
Satifiketi Yotsatira Malamulo	Chidutswa chimodzi	Chidutswa chimodzi

Kuyenda kwa Ntchito

Gawo 1: Kukonzekera Chitsanzo

1. Chida choyesera chingachitike pogwiritsa ntchito magazi athunthu, seramu kapena plasma. Perekani lingaliro losankha seramu kapena plasma ngati chitsanzo choyesera. Ngati mwasankha magazi athunthu ngati chitsanzo choyesera, chiyenera kugwiritsidwa ntchito limodzi ndi choyezera magazi.

2. Yesani chitsanzo pa khadi loyesera nthawi yomweyo. Ngati mayeso sangathe kutha nthawi yomweyo, seramu ndi plasma ziyenera kusungidwa kwa masiku 7 pa 2 ~ 8℃ kapena kusungidwa pa -20℃ kwa miyezi 6 (chitsanzo cha magazi onse chiyenera kusungidwa kwa masiku atatu pa 2 ~ 8℃) mpaka chikayesedwe.

3. Zitsanzo ziyenera kubwezeretsedwa kutentha kwa chipinda musanayesedwe. Zitsanzo zozizira ziyenera kusungunuka kwathunthu ndikusakanizidwa bwino musanayesedwe, kupewa kuzizira mobwerezabwereza ndi kusungunuka.

4. Pewani kutentha zitsanzo, zomwe zingayambitse kusweka kwa magazi ndi kuwonongeka kwa mapuloteni. Ndikoyenera kupewa kugwiritsa ntchito chitsanzo chosweka kwambiri cha magazi. Ngati chitsanzo chikuoneka kuti chasweka kwambiri, chitsanzo china chiyenera kupezedwa ndikuyesedwa.

Gawo 2: Kuyesa

1. Chonde werengani bukuli mosamala musanayese, bwezeretsani chitsanzo, khadi loyesera ndi chitsanzo cha magazi kuti chifike kutentha kwa chipinda ndikulemba manambala pa khadi. Perekani malangizo oti mutsegule thumba la zojambulazo mutabwerera kutentha kwa chipinda ndikugwiritsa ntchito khadi loyesera nthawi yomweyo.

2. Ikani khadi loyesera patebulo loyera, loyikidwa mopingasa.

Kwa chitsanzo cha Seramu kapena Plasma:

Gwirani chotsitsacho moyimirira ndikusamutsa madontho atatu a seramu kapena plasma (pafupifupi 80 L, Pipette ingagwiritsidwe ntchito pazidzidzidzi) ku chitsime cha chitsanzo, ndikuyambitsa nthawi. Onani chithunzi pansipa.

Kwa chitsanzo cha magazi athunthu:

Gwirani chotsitsacho molunjika ndikusamutsa madontho atatu a magazi athunthu (pafupifupi 80 L) ku chitsime cha chitsanzo, kenako onjezerani dontho limodzi la chosungunula cha chitsanzo (pafupifupi 40 L), ndikuyambitsa nthawi. Onani chithunzi pansipa.

Gawo 3: Kuwerenga

Mu mphindi 10 mpaka 30, pezani zotsatira za theka-quantitative malinga ndi khadi yodziwika bwino ya colorimetric ndi maso.

Kutanthauzira zotsatira

Zolondola: Mzere umodzi wofiira wofiirira umawonekera pa mzere wolamulira (C). Ponena za zotsatira zolondola, mutha kupeza kuchuluka kwa maso pogwiritsa ntchito khadi yokhazikika ya colorimetric:

Mphamvu ya Mtundu vs Kuchuluka kwa Ma Reference

Mphamvu ya Utoto	Kukhazikika kwa Reference (ng / ml)
-	＜0.5
+ -	0.5~1
+	1 mpaka 5
+ +	5~15
+ + +	15~30
+ + + +	30~50
+ + + +	＞50

Chosalondola: Palibe mzere wofiira wofiirira womwe umawonekera pa mzere wolamulira (C). Izi zikutanthauza kuti magwiridwe antchito ena ayenera kuti anali olakwika kapena khadi yoyesera yakhala yolakwika kale. Pachifukwa ichi chonde werengani bukuli mosamala kachiwiri, ndikuyesanso ndi kaseti yatsopano yoyesera. Ngati vuto lomweli lachitikanso, muyenera kusiya kugwiritsa ntchito gulu ili la zinthu nthawi yomweyo ndikulankhula ndi omwe akukupatsani.

Zambiri Zokhudza Oda

Dzina la Chinthu	Mphaka. Ayi	Kukula	Chitsanzo	Moyo wa Shelufu	Kusinthasintha ndi Kusinthasintha kwa Kutentha.
Chida Choyesera Mwachangu cha Troponin I cha Mtima (Lateral Chromatography)	B032C-01	Chiyeso chimodzi/zida	S/P/WB	Miyezi 24	2-30℃
	B032C-25	Mayeso 25/zida	S/P/WB	Miyezi 24	2-30℃