S. Typhi / Paratyphi Combo Antigen Rapid Test Kit (Immunochromatographic Assay) Chithunzi Chowonetsedwa

S. Typhi / Paratyphi Combo Antigen Rapid Test Kit (Kuyesa kwa Immunochromatographic)

Kufotokozera Kwachidule:

Chitsanzo	Chimbudzi	Mtundu	Kaseti
Kuzindikira	Typhi (93.10%) Paratyphi (93.41%)	Kufotokozera Mwapadera	Typhi (92.53%) Paratyphi (94.59%)
Kusinthasintha ndi Kusinthasintha kwa Kutentha.	2-30℃ / 36-86℉	Nthawi Yoyesera	Mphindi 10-20
Kufotokozera	Chiyeso chimodzi/Chida; Mayeso 5/Chida; Mayeso 25/Chida

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Ntchito Yoyenera:

S. Typhi/Paratyphi Combo Antigen Rapid Test Kit (Immunochromatographic Assay) ndi mayeso othamanga, oyenda pang'onopang'ono, omwe amadziwikanso kuti lateral flow immunochromatographic assay, omwe cholinga chake ndi kuzindikira bwino ma antigen a S. Typhi ndi Paratyphi m'mabotolo a ndowe kuchokera kwa odwala. Zotsatira za S. Typhi/Paratyphi Combo Antigen Rapid Test Kit ziyenera kutanthauziridwa mogwirizana ndi kuwunika kwa wodwala ndi njira zina zodziwira matenda.

Mfundo Zoyesera:

S. Typhi/Paratyphi Combo Antigen Rapid Test Kit (Immunochromatographic Assay) ndi njira yoyesera ya chromatographic flow flow. Ili ndi mizere itatu yopangidwa kale, mzere wa "T1" S. Typhi Test, mzere wa "T2" Paratyphi Test ndi mzere wa "C" Control pa nembanemba ya nitrocellulose. Ma antibodies a monoclonal anti-S. Typhi ndi anti-Paratyphi akuphimbidwa pa dera la mzere woyesera ndipo ma antibodies a Goat anti-chicken IgY akuphimbidwa pa dera lowongolera. Chitsanzocho chikakonzedwa ndikuwonjezedwa pachitsime cha chitsanzo, ma antigen a S. Typhi/Paratyphi omwe ali mu chitsanzocho amalumikizana ndi ma complexes a S. Typhi/Paratyphi Antibody-labeled conjugate omwe amapanga antigen-antibody color particles. Ma complexes amasuntha pa nembanemba ya nitrocellulose kudzera mu capillary action mpaka mzere woyesera, komwe amagwidwa ndi ma antibodies a monoclonal anti-S. Typhi/Paratyphi a mbewa. Mzere wa T1 wofiirira umawonekera pazenera la zotsatira ngati ma antigen a S. Typhi alipo mu chitsanzo ndipo mphamvu yake imadalira kuchuluka kwa antigen ya S. Typhi. Mzere wa T2 wofiirira umawonekera pazenera la zotsatira ngati ma antigen a Paratyphi alipo mu chitsanzo ndipo mphamvu yake imadalira kuchuluka kwa antigen ya Paratyphi. Ngati ma antigen a S.Typhi/Paratyphi omwe ali mu chitsanzocho palibe kapena ali pansi pa malire odziwika, palibe gulu looneka la utoto mu mzere wa Test (T1 ndi T2) wa chipangizocho. Izi zikusonyeza zotsatira zoyipa. Mzere woyesera kapena mzere wolamulira sizimawonekera mu zenera la zotsatira musanagwiritse ntchito chitsanzocho. Mzere wowongolera wowoneka umafunika kuti uwonetse kuti zotsatira zake ndi zovomerezeka.

Zamkatimu Zazikulu

Zigawo zomwe zaperekedwa zalembedwa patebulo.

Chigawo cha REF REF	B033C-01	B033C-05	B033C-25
Kaseti Yoyesera	Mayeso 1	Mayeso 5	Mayeso 25
Buffer	Botolo limodzi	Botolo 5	Mabotolo 25/2
Chikwama Choyendera cha Zitsanzo	Chidutswa chimodzi	Ma PC 5	Ma PC 25
Malangizo Ogwiritsira Ntchito	Chidutswa chimodzi	Ma PC 5	Ma PC 25
Satifiketi Yotsatira Malamulo	Chidutswa chimodzi	Chidutswa chimodzi	Chidutswa chimodzi

Kuyenda kwa Ntchito

Gawo 1: ChitsanzoKukonzekera

1. Sonkhanitsani zitsanzo za ndowe m'zidebe zoyera komanso zosatulutsa madzi.
2. Kunyamula ndi Kusunga Zitsanzo: Zitsanzo zitha kusungidwa kutentha kwa chipinda kwa maola 8 kapena kusungidwa mufiriji pa 36°F mpaka 46°F (2°C mpaka 8°C) kwa maola 96.
3. Zitsanzo za ndowe zomwe zimasungidwa mufiriji zimatha kusungunuka mpaka kawiri pa -10°C kapena pansi pake. Ngati mukugwiritsa ntchito zitsanzo zozizira, sungunulani kutentha kwa chipinda. Musalole zitsanzo za ndowe kukhalabe mu chisakanizo cha diluent kwa maola oposa awiri.

Gawo 2: Kuyesa

1. Chonde werengani malangizo mosamala musanayese. Musanayese, lolani makaseti oyesera, yankho la zitsanzo ndi zitsanzo zikhale zofanana ndi kutentha kwa chipinda (15-30℃ kapena 59-86 madigiri Fahrenheit).

2. Chotsani kaseti yoyesera kuchokera mu thumba la zojambulazo ndikuyiyika pamalo athyathyathya.

3. Tulutsani botolo la chitsanzo, gwiritsani ntchito ndodo yolumikizira yomwe yaikidwa pa chivundikiro kuti mutumize kachidutswa kakang'ono ka chitsanzo cha ndowe (3-5 mm m'mimba mwake; pafupifupi 30-50 mg) mu botolo la chitsanzo lomwe lili ndi buffer yokonzekera chitsanzo.

4. Bwezerani ndodo mu botolo ndikulilimbitsa bwino. Sakanizani chitsanzo cha ndowe ndi chosungira bwino pogwedeza botolo kangapo ndikusiya chubucho kwa mphindi ziwiri.

5. Tsegulani nsonga ya botolo la chitsanzo ndikugwirizira botolo moyimirira pamwamba pa chitsime cha chitsanzo cha Kaseti, perekani madontho atatu (100 -120μL) a chitsanzo cha ndowe chochepetsedwa ku chitsime cha chitsanzo.

Gawo 3: Kuwerenga

Werengani zotsatira mu mphindi 15-20. Nthawi yofotokozera zotsatira si yoposa mphindi 20

Kutanthauzira zotsatira

1. Zotsatira Zabwino za S. Typhi

Mizere yamitundu imawonekera pa mzere woyesera (T1) ndi mzere wolamulira (C). Izi zikusonyeza zotsatira zabwino za ma antigen a S. Typhi mu chitsanzo.

2. Zotsatira Zabwino za Paratyphi

Mizere yamitundu imawonekera pa mzere woyesera (T2) ndi mzere wowongolera (C). Izi zikusonyeza zotsatira zabwino za ma antigen a Paratyphi mu chitsanzo.

3. Zotsatira Zabwino za S. Typhi ndi Paratyphi

Mizere yamitundu imawonekera pa mzere woyesera (T1), mzere woyesera (T2) ndi mzere wolamulira (C). Izi zikusonyeza zotsatira zabwino za ma antigen a S. Typhi ndi Paratyphi mu chitsanzocho.

4. Zotsatira Zoyipa

Mzere wamitundu umawonekera pamzere wowongolera (C) wokha. Zimasonyeza kuti kuchuluka kwa ma antigen a S. Typhi kapena Paratyphi kulibe kapena kulibe malire odziwika a mayesowo.

5. Zotsatira Zolakwika

Palibe mzere wooneka wamitundu womwe umawonekera pamzere wowongolera mutachita mayeso. Malangizo mwina sanatsatidwe bwino kapena mayesowo mwina awonongeka. Ndikofunikira kuti chitsanzocho chiyesedwenso.

Zambiri Zokhudza Oda

Dzina la Chinthu	Mphaka. Ayi	Kukula	Chitsanzo	Moyo wa Shelufu	Kusinthasintha ndi Kusinthasintha kwa Kutentha.
S. Typhi/Paratyphi Combo Antigen Rapid Test Kit (Kuyesa kwa Immunochromatographic)	B033C-01	Chiyeso chimodzi/zida	Chimbudzi	Miyezi 24	2-30℃
	B033C-05	Mayeso 5/zida
	B033C-25	Mayeso 25/zida