Leishmania IgG/IgM Antibody Test Kit (Immunochromatographic Assay)
Zambiri zamalonda
Cholinga Chogwiritsidwa Ntchito
Mankhwalawa ndi oyenera kuwunika kwachipatala kwa seramu / plasma / zitsanzo zamagazi athunthu kuti azindikire ma antibodies motsutsana ndi Leishmania.Ndi mayeso osavuta, ofulumira komanso osagwiritsa ntchito zida kuti apeze matenda a kala-azar oyambitsidwa ndi Leishmania.
Mfundo Yoyesera
Izi ndi lateral flow chromatographic immunoassay.Kaseti yoyesera ili ndi: 1) burgundy colored conjugate pad yomwe ili ndi antigen rK39 antigen conjugated ndi colloid gold (Leishmania conjugates) ndi kalulu IgG-golide conjugates;2) mzere wa membrane wa nitrocellulose wokhala ndi magulu awiri oyesera (M ndi G band) ndi gulu lowongolera (C band).
Zamkatimu Zazikulu
Zigawo zoperekedwa zandandalikidwa patebulo.
| Zipangizo / zoperekedwa | Kuchuluka (1 Mayeso/Kiti) | Kuchuluka(5Mayeso/Kiti)
| Kuchuluka(25Mayeso/Kit)
|
| Zida Zoyesera | 1 mayeso | 5 mayeso | 25 mayesero |
| Bafa | 1 botolo | 5 botolo | 25/2 mabotolo |
| Chotsitsa | 1 chidutswa | 5 pcs | 25 pcs |
| Chikwama Chonyamula Chitsanzo | 1 chidutswa | 5 pcs | 25 pcs |
| Lancet yotayika | 1 chidutswa | 5 pcs | 25 pcs |
| Malangizo Ogwiritsa Ntchito | 1 chidutswa | 1 chidutswa | 1 chidutswa |
| Satifiketi Yogwirizana | 1 chidutswa | 1 chidutswa | 1 chidutswa |
Operation Flow
Gawo 1: Sample
Sungani Seramu yaumunthu / Plasma / Magazi Athunthu moyenera.
Gawo 2: Kuyesa
Chonde werengani malangizo mosamala musanayese.Musanayesere, lolani zida zoyeserera, yankho lachitsanzo ndi zitsanzo kuti zikhale zoyezera kutentha (15-30 ℃ kapena 59-86 madigiri Fahrenheit).
1.Chotsani chubu chochotsa muzitsulo ndi bokosi loyesera kuchokera mu thumba la filimu pong'amba mphako.Ikani iwo pa ndege yopingasa.
2.Tsegulani khadi loyendera thumba la aluminium zojambulazo.Chotsani khadi yoyesera ndikuyiyika mopingasa patebulo.
3.Gwiritsani ntchito pipette yotayika, tumizani dontho limodzi (pafupifupi 20μL) magazi a chala / kapena 4μL seramu / kapena 4μL plasma / kapena 4μL magazi athunthu mu chitsanzo chabwino pa kaseti yoyesera.
4.Tsegulani buffer chubu.Ikani madontho atatu (pafupifupi 80 μL) oyezera muyeso woyezera wozungulira wozungulira.Yambani kuwerenga.
Gawo 3: Kuwerenga
Werengani zotsatira pa mphindi 5-10.Zotsatira pakadutsa mphindi 10 ndizolakwika.
Kutanthauzira zotsatira
Zotsatira zoyipa
Ngati mzere wowongolera wamtundu wa C ukuwonekera ndipo mizere yodziwikiratu G ndi M sichikuwonetsa
Zotsatira Zabwino
1. Onse mzere wowongolera khalidwe C ndi mzere wodziwikiratu M ukuwoneka= antibody ya Leishmania IgM imapezeka, ndipo zotsatira zake zimakhala zabwino kwa antibody ya IgM.
2. Onse mzere wowongolera khalidwe C ndi mzere wodziwikiratu G ukuwoneka= antibody ya Leishmania IgG imadziwika ndipo zotsatira zake zimakhala zabwino kwa antibody ya IgG.
3. Onse mzere wowongolera khalidwe C ndi mizere yodziwikiratu G ndi M ikuwonekera=ma antibodies a Leishmania IgG ndi IgM.zizindikirika, ndipo zotsatira zake zimakhala zabwino kwa ma antibodies a IgG ndi IgM.
Zotsatira zosalondola
Mzere wowongolera khalidwe C sungathe kuwonedwa, zotsatira zidzakhala zosavomerezeka mosasamala kanthu kuti mzere woyesera umasonyeza, ndipo mayesero ayenera kubwerezedwa.
Kuitanitsa Zambiri
| Dzina lazogulitsa | Mphaka.Ayi | Kukula | Chitsanzo | Shelf Life | Trans.ndi Sto.Temp. |
| Leishmania IgG/IgM Antibody Test Kit (Immunochromatographic Assay) | B020C-01 | 1 mayeso / zida | Seramu / Plasma / Magazi Athunthu | 18 Miyezi | 2-30 ℃ / 36-86 ℉ |
| B020C-05 | 5 mayeso / zida | ||||
| B020C-25 | 25 mayeso / zida |
