Ntchito ya Monoclonal Antibody
Antigen ya monoclonal, yopangidwa ndi gulu limodzi la B cell, imakhala ndi ma homogeneity kwambiri omwe amalunjika pa antigen epitope.Monoclonal antibody ili ndi zabwino zambiri, kuphatikiza kuyera kwambiri, kukhudzika, komanso kutsimikizika.Ma Hybridoma amapangidwa kudzera mu kuphatikizika kwa ma lymphocyte a B omwe amapanga ma antibodies enieni, okhala ndi ma cell a myeloma omwe amakhala nthawi yayitali.Bioantibody imagwiritsa ntchito ukadaulo wophatikizika kwambiri womwe umagwira ntchito kuwirikiza ka 20 kuposa njira wamba wamba.Kuphatikiza apo, imagwiritsa ntchito ukadaulo wowunikira ma protein a microarray kuti azindikire ma antibodies a monoclonal omwe amawonetsa kutsimikizika kwakukulu, kuyanjana, komanso magwiridwe antchito motsutsana ndi ma epitopes ena.
| Zinthu Zothandizira | Zamkatimu Zoyeserera | Nthawi Yotsogolera (Sabata) |
| Kukonzekera kwa Antigen | 1. Makasitomala amapereka antigen2. Maantijeni amadzimadzi amakonzekera antigen | / |
| Katemera wa mbewa | Katemera wa BALB/c mbewa, kusonkhanitsa seramu ndi kusanthula kwa ELISA | 4 |
| Kuphatikizika kwa ma cell ndikuwunika | Kuphatikizika kwa ma splenocyte a mbewa ndi ma cell a myeloma, kuwunika kwa HAT | 2 |
| Kukhazikika kwa mzere wama cell | Kuphatikizidwa kwa ma clones owoneka bwino | 3 |
| Chizindikiritso cha isotype cha antibody | Kuzindikiritsa ma cell line subtypes | 1 |
| Makulitsidwe ang'onoang'ono | Makulitsidwe opanda serum | 2 |
| Kukulitsa kwakukulu ndi kuyeretsa | 200mL wopanda seramu makulitsidwe ndi kuyeretsa | 1 |
