Prokaryotic Protein Expression
Dongosolo la mawu a prokaryotic E. coli limavomerezedwa ndi anthu ambiri ngati njira yotsika mtengo kwambiri, yokhwima mwaukadaulo, komanso yogwiritsidwa ntchito mofala pofotokozera zomanga thupi.Ku Bioantibody, tadzipereka kupatsa makasitomala athu ntchito zambiri, zoyimitsa kamodzi kuyambira kaphatikizidwe ka majini mpaka kafotokozedwe ka mapuloteni ndi kuyeretsa.Ntchito zathu zikuphatikiza kukhathamiritsa kwa ma codon kwaulere komanso kugwiritsa ntchito ukadaulo wathu wa eni kuti tithane ndi zovuta zilizonse zokhudzana ndi kutsika komanso kusasunthika komwe kungabwere panthawi yonse yofotokozera ndi kuyeretsa.Makasitomala athu amangofunika kupereka jini kapena ma amino acid a protein ndipo titha kupereka mapuloteni apamwamba kwambiri mu FAST ngati milungu itatu.Kuphatikiza apo, Bioantibody imapereka ntchito zochotsa endotoxin ndikuyika ma tagging mogwirizana ndi zosowa za makasitomala athu.Ndife odzipereka kupereka zotsatira ndikulonjeza kuti sitilipira chindapusa ngati puloteni yomaliza sinafotokozedwe.
| Zinthu Zothandizira | Zinthu Zoyeserera | Nthawi Yotsogolera (BD) |
| Gene Synthesis | Kukhathamiritsa kwa Codon, kaphatikizidwe ka jini ndi subcloning. | 5-10 |
| Chizindikiritso cha Mawu ndi Kusanthula kwa Solubility | 1. Kusintha ndi makulitsidwe, kuzindikira mawu ndi SDS-PAGE.2. Kusanthula kwamadzimadzi, SDS-PAGE ndi kuzindikira kwa WB | 10 |
| Kuyika kwakukulu ndi kuyeretsa, mapuloteni omaliza (kuyera> 85%, 90%, 95%) ndi lipoti loyesera | Kuyeretsedwa kwa Affinity (Ni column, MBP, GST) |
Ngati jini yapangidwa mkatiBioantibody, plasmid yopangidwa idzaphatikizidwa muzoperekedwa.