Insect Cell Protein Expression
The insect cell expression system ndi njira yomwe imagwiritsidwa ntchito kwambiri pofotokozera mapuloteni akuluakulu.Poyerekeza ndi ma cell a mammalian, chikhalidwe cha tizilombo toyambitsa matenda ndi chosavuta ndipo sichifuna CO2.Baculovirus ndi mtundu wa kachilombo ka DNA kakang'ono kawiri komwe kumakhala ndi tizilombo toyambitsa matenda.Lili ndi zamoyo zambiri, silimapha tizilombo ta msana, komanso silivulaza anthu ndi ziweto.sf9, yomwe imagwiritsidwa ntchito kwambiri ngati cell host, imapezeka mu planktonic kapena kutsatira chikhalidwe.sf9 ndiyoyenera kufotokozera kwakukulu, ndipo itha kugwiritsidwa ntchito pokonza ndikusintha mapuloteni monga phosphorylation, glycosylation, ndi acylation.Dongosolo la ma cell a tizilombo litha kugwiritsidwanso ntchito pofotokozera majini angapo, komanso limatha kuwonetsa mapuloteni oopsa monga antimicrobial peptides.
| Zinthu Zothandizira | Nthawi Yotsogolera (BD) |
| Kukhathamiritsa kwa Codon, kaphatikizidwe ka jini ndi subcloning | 5-10 |
| P1 generation virus incubation and small scale expression | 10-15 |
| P2 generation virus incubation, kufotokoza kwakukulu ndi kuyeretsedwa, kupereka mapuloteni oyeretsedwa ndi lipoti loyesera |