Yeast Cell Protein Expression
Dongosolo la yisiti ndi njira yomwe imagwiritsidwa ntchito kwambiri pofotokozera mapuloteni a eukaryotic, chifukwa cha kuphweka kwake pakulima, kukwanitsa, komanso kugwira ntchito mosavuta.Pakati pa mitundu yosiyanasiyana ya yisiti, Pichia pastoris ndi omwe amadziwika kwambiri, chifukwa amathandizira kufotokoza kwa mapuloteni a intracellular ndi extracellular.Dongosololi limathandiziranso kusinthidwa pambuyo pomasulira, monga phosphorylation ndi glycosylation, zomwe zimapangitsa kuti pakhale mawonekedwe apadera a eukaryotic okhala ndi maubwino ambiri.
| Zinthu Zothandizira | Nthawi Yotsogolera (BD) |
| Kukhathamiritsa kwa Codon, kaphatikizidwe ka jini ndi subcloning | 5-10 |
| Kuwunika kwabwino kwa ma clone | 10-15 |
| Mawu ang'onoang'ono | |
| Kufotokozera kwakukulu (200ML) ndi kuyeretsedwa, zomwe zimaperekedwa zimaphatikizapo mapuloteni oyeretsedwa ndi lipoti loyesera |
Ngati jini yapangidwa mu Bioantibody, plasmid yopangidwayo idzaphatikizidwa muzoperekedwa.