Zina zambiri
Alpha-fetoprotein (AFP) amatchulidwa kuti ndi membala wa jini ya albuminoid superfamily yomwe ili ndi albumin, AFP, vitamini D (Gc) mapuloteni, ndi alpha-albumin.AFP ndi glycoprotein wa 591 amino acid ndi gawo lazakudya.AFP ndi imodzi mwamapuloteni okhudzana ndi embryo ndipo ndi puloteni yodziwika bwino ya seramu kuyambira pachiyambi cha moyo waubwana wa mwezi umodzi, pamene albumin ndi transferrin zimakhalapo pang'ono.Imapangidwa koyamba mwa munthu ndi yolk sac ndi chiwindi (miyezi 1-2) ndipo makamaka m'chiwindi.Kuchepa kwa AFP kumapangidwa ndi thirakiti la GI la lingaliro laumunthu.Zatsimikiziridwa kuti AFP ikhoza kuwonekeranso mu seramu muzowonjezereka mu moyo wachikulire mogwirizana ndi njira zobwezeretsa bwino komanso ndi kukula koyipa.Alpha-fetoprotein (AFP) ndi chizindikiro cha hepatocellular carcinoma (HCC), teratoblastomas, ndi neural tube defect (NTD).
Awiri Malangizo | CLIA (Kujambula-Kuzindikira): 3C8-6 ~ 11D1-2 8A3-7 ~ 11D1-2 |
Chiyero | > 95%, yotsimikiziridwa ndi SDS-PAGE |
Kupanga kwa Buffer | PBS, pH7.4. |
Kusungirako | Sungani mumkhalidwe wosabala pa -20℃ku -80℃atalandira. Ndibwino kuti aliquot mapuloteniwo muchuluke kakang'ono kuti asungidwe bwino. |
Dzina lazogulitsa | Mphaka.Ayi | Clone ID |
AFP | Chithunzi cha AB0069-1 | Chithunzi cha 11D1-2 |
AB0069-2 | 3C8-6 | |
Mtengo wa AB0069-3 | 8A3-7 |
Chidziwitso: Bioantibody imatha makonda malinga ndi zosowa zanu.
1.Mizejewski GJ.(2001) Mapangidwe a Alpha-fetoprotein ndi Ntchito: Kufunika kwa Isoforms, Epitopes, ndi Conformational Variants.Exp Biol Med.226 (5): 377-408.
2.Tomasi TB, ndi al.(1977) Mapangidwe ndi Ntchito ya Alpha-Fetoprotein.Ndemanga Yamankhwala Yapachaka.28:453-65 .
3.Leguy MC, et al.(2011) Kuunika kwa AFP mu amniotic fluid: kuyerekeza njira zitatu zodzichitira.Ann Biol Clin.69(4): 441-6.