Cholinga Chogwiritsidwa Ntchito
The Chagas IgG Antibody Test Kit (Immunochromatographic Assay) ndi lateral flow chromatographic immunoassay pofuna kudziwa bwino za IgG anti-Trypanosoma cruzi (T. cruzi) mu seramu yaumunthu, plasma kapena magazi athunthu.Amapangidwa kuti azigwiritsidwa ntchito ngati mayeso owunika komanso ngati chithandizo cha matenda a T. openga.
Mfundo Yoyesera
Chagas IgG Antibody Test Kit ndi lateral flow chromatographic immunoassay kutengera mfundo ya indirect immunoassay.Padi yolumikizira yamitundu yokhala ndi Mapuloteni ophatikizidwa ndi golide wa colloid (Mapuloteni conjugates);Mzere wa membrane wa nitrocellulose wokhala ndi bandi yoyesera (T band) ndi gulu lowongolera (C band).Gulu la T limayikidwa kale ndi ma antigen a T. cruzi, ndipo gulu la C limayikidwa kale ndi ma antibodies a antiProtein.
Gawo REF/REF | B016C-01 | B016C-05 | B016C-25 |
Mayeso Kaseti | 1 mayeso | 5 mayeso | 25 mayesero |
Bafa | 1 botolo | 5 botolo | 25 botolo |
Chotsitsa | 1 chidutswa | 5 pcs | 25 pcs |
Chikwama Chonyamula Chitsanzo | 1 chidutswa | 5 pcs | 25 pcs |
Lancet yotayika | 1 chidutswa | 5 pcs | 25 pcs |
Malangizo Ogwiritsa Ntchito | 1 chidutswa | 1 chidutswa | 1 chidutswa |
Satifiketi Yogwirizana | 1 chidutswa | 1 chidutswa | 1 chidutswa |
Gawo 1: Sample
Sungani Seramu yaumunthu / Plasma / Magazi Athunthu moyenera.
Gawo 2: Kuyesa
1. Chotsani chubu chochotsa muzitsulo ndi bokosi loyesera kuchokera m'thumba la filimu pong'amba mphako.Ikani iwo pa ndege yopingasa.
2. Tsegulani khadi loyendera thumba la aluminium zojambulazo.Chotsani khadi yoyesera ndikuyiyika mopingasa patebulo.
3. Gwiritsani ntchito pipette yotayika, tumizani 40μL seramu/ kapena plasma/kapena 40μ L magazi athunthu muchitsime chapa kaseti yoyesera.
3. Tsegulani buffer chubu pokhota kuchokera pamwamba.Ikani madontho atatu (pafupifupi 80 μL) a buffer mu choyesa choyezera chozungulira bwino.Yambani kuwerenga.
Gawo 3: Kuwerenga
Pambuyo pa mphindi 15, werengani zotsatira zake mowonekera.(Zindikirani: chitaniOSATIwerengani zotsatira pambuyo pa mphindi 10!)
1.Zotsatira Zabwino
Ngati zonse ziwiri zowongolera mtundu C mzere ndi mzere wozindikira T ziwoneka, ndipo zotsatira zake zimakhala zabwino kwa Chagas Antibody.
2. Zotsatira Zoipa
Ngati mzere wa C wowongolera khalidwe ukuwonekera ndipo mzere wa T wodziwikiratu sukuwonetsa mtundu, zimasonyeza kuti palibe Chagas Antibody mu chitsanzocho.
3. Zotsatira zosalondola
Palibe gulu lowoneka bwino lomwe likuwoneka pamzere wowongolera mutatha kuyesa.Kusakwanira kwachitsanzo kwachitsanzo kapena njira zolakwika ndizo zifukwa zomwe zimalepheretsa mzere wowongolera.Unikaninso njira yoyeserera ndikubwereza kuyesanso pogwiritsa ntchito chida chatsopano choyesera.
Dzina lazogulitsa | Mphaka.Ayi | Kukula | Chitsanzo | Shelf Life | Trans.ndi Sto.Temp. |
Chagas IgG Antibody test kit (Immunochromatographic Assay) | B016C-001 | 1 mayeso / zida | Seramu / Plasma / Magazi Athunthu | 18 Miyezi | 2-30 ℃ / 36-86 ℉ |
B016C-05 | 5 mayeso / zida | ||||
B016C-25 | 25 mayeso / zida |